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Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3α,4α-diaza-s-indacene (Bodipy)-FL-510, d...
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| Auteurs principaux: | , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
2004
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC397410/ https://ncbi.nlm.nih.gov/pubmed/15064393 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401138101 |
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