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Photocleavage of a 2-nitrobenzyl linker bridging a fluorophore to the 5′ end of DNA
Three single-stranded DNA molecules of different lengths were synthesized and characterized, each containing a fluorescent dye (6-carboxyfluorescein) connected to the 5′ end via a photocleavable 2-nitrobenzyl linker and a biotin moiety at the 3′ end. UV irradiation (λ ≈ 340 nm) of solutions containi...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
The National Academy of Sciences
2003
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC141008/ https://ncbi.nlm.nih.gov/pubmed/12515854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.242729099 |
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