Photocleavage of a 2-nitrobenzyl linker bridging a fluorophore to the 5′ end of DNA

Three single-stranded DNA molecules of different lengths were synthesized and characterized, each containing a fluorescent dye (6-carboxyfluorescein) connected to the 5′ end via a photocleavable 2-nitrobenzyl linker and a biotin moiety at the 3′ end. UV irradiation (λ ≈ 340 nm) of solutions containi...

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Publicado en:	Proc Natl Acad Sci U S A
Autores principales:	Bai, Xiaopeng, Li, Zengmin, Jockusch, Steffen, Turro, Nicholas J., Ju, Jingyue
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	National Academy of Sciences 2003
Materias:	Physical Sciences
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC141008/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12515854/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.242729099
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