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Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry

We report here the design, synthesis and evaluation of a novel photocleavable (PC) biotinylated nucleotide analog, dUTP-PC-Biotin, for DNA polymerase extension reaction to isolate DNA products for mass spectrometry (MS) analysis. This nucleotide analog has a biotin moiety attached to the 5-position...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphovsmän: Bai, Xiaopeng, Kim, Sobin, Li, Zengmin, Turro, Nicholas J., Ju, Jingyue
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2004
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373325/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14744978/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkh198
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