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Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry
We report here the design, synthesis and evaluation of a novel photocleavable (PC) biotinylated nucleotide analog, dUTP-PC-Biotin, for DNA polymerase extension reaction to isolate DNA products for mass spectrometry (MS) analysis. This nucleotide analog has a biotin moiety attached to the 5-position...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373325/ https://ncbi.nlm.nih.gov/pubmed/14744978 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh198 |
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