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Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3α,4α-diaza-s-indacene (Bodipy)-FL-510, d...

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Detalhes bibliográficos
Main Authors: Seo, Tae Seok, Bai, Xiaopeng, Ruparel, Hameer, Li, Zengmin, Turro, Nicholas J., Ju, Jingyue
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC397410/
https://ncbi.nlm.nih.gov/pubmed/15064393
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401138101
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