Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3α,4α-diaza-s-indacene (Bodipy)-FL-510, d...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Seo, Tae Seok, Bai, Xiaopeng, Ruparel, Hameer, Li, Zengmin, Turro, Nicholas J., Ju, Jingyue
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 2004
Schlagworte:	Physical Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397410/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15064393/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0401138101
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