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A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity
In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease–exonuclease–...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2014
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3950723/ https://ncbi.nlm.nih.gov/pubmed/24170810 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt972 |
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