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An assay to measure the affinity of proteins for microtubules by quantitative fluorescent microscopy
We report a fluorescence-based assay for measuring the affinity of microtubule binding proteins for microtubules. The affinity of any fluorescently tagged protein for taxol-stabilized microtubules can be measured with this assay. We describe the assay and provide a detailed protocol. Using this assa...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3035983/ https://ncbi.nlm.nih.gov/pubmed/21146493 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.12.009 |
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