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Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis

We have assessed the efficacy of the recently developed CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system for genome modification in the amphibian Xenopus tropicalis. As a model experiment, targeted mutations of the tyrosinase gene were verified, showing...

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Detalhes bibliográficos
Main Authors: Nakayama, Takuya, Fish, Margaret B., Fisher, Marilyn, Oomen-Hajagos, Jamina, Thomsen, Gerald H., Grainger, Robert M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3947545/
https://ncbi.nlm.nih.gov/pubmed/24123613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvg.22720
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