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Simple, Fast, Tissue-specific Bacterial Artificial Chromosome Transgenesis in Xenopus

We have developed a method of injecting Bacterial Artificial Chromosome (BAC) DNA into Xenopus embryos that is simple and efficient, and results in consistent and tissue-specific expression of transgenes cloned into BAC vectors. Working with large pieces of DNA, as can be accommodated by BACs, is ne...

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Detalhes bibliográficos
Main Authors: Fish, Margaret B., Nakayama, Takuya, Grainger, Robert M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3870158/
https://ncbi.nlm.nih.gov/pubmed/22084035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvg.20819
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