Dynamics of Translocation and Substrate Binding in Individual Complexes Formed with Active Site Mutants of Φ29 DNA Polymerase

The Φ29 DNA polymerase (DNAP) is a processive B-family replicative DNAP. Fluctuations between the pre-translocation and post-translocation states can be quantified from ionic current traces, when individual Φ29 DNAP-DNA complexes are held atop a nanopore in an electric field. Based upon crystal stru...

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Bibliographic Details
Main Authors: Dahl, Joseph M., Wang, Hongyun, Lázaro, José M., Salas, Margarita, Lieberman, Kate R.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2014
Subjects:
Enzymology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3945302/
https://ncbi.nlm.nih.gov/pubmed/24464581
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.535666
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