Direct Observation of Translocation in Individual DNA Polymerase Complexes

Complexes of phi29 DNA polymerase and DNA fluctuate on the millisecond time scale between two ionic current amplitude states when captured atop the α-hemolysin nanopore in an applied field. The lower amplitude state is stabilized by complementary dNTP and thus corresponds to complexes in the post-tr...

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Main Authors: Dahl, Joseph M., Mai, Ai H., Cherf, Gerald M., Jetha, Nahid N., Garalde, Daniel R., Marziali, Andre, Akeson, Mark, Wang, Hongyun, Lieberman, Kate R.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Biochemistry and Molecular Biology 2012
Ábhair:
Enzymology
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3339981/
https://ncbi.nlm.nih.gov/pubmed/22378784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.338418
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