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Specific Nucleotide Binding and Rebinding to Individual DNA Polymerase Complexes Captured on a Nanopore

Nanoscale pores are a tool for single molecule analysis of DNA or RNA processing enzymes. Monitoring catalytic activity in real time using this technique requires that these enzymes retain function while held atop a nanopore in an applied electric field. Using an α-hemolysin nanopore, we measured th...

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Detalhes bibliográficos
Main Authors: Hurt, Nicholas, Wang, Hongyun, Akeson, Mark, Lieberman, Kate R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2666922/
https://ncbi.nlm.nih.gov/pubmed/19275265
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja809663f
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