Tethered Particle Motion Reveals that LacI·DNA Loops Coexist with a Competitor-Resistant but Apparently Unlooped Conformation

The lac repressor protein (LacI) efficiently represses transcription of the lac operon in Escherichia coli by binding to two distant operator sites on the bacterial DNA and causing the intervening DNA to form a loop. We employed single-molecule tethered particle motion to observe LacI-mediated loop...

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Bibliographic Details
Main Authors: Revalee, Joel D., Blab, Gerhard A., Wilson, Henry D., Kahn, Jason D., Meiners, Jens-Christian
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2014
Subjects:
Proteins and Nucleic Acids
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3944957/
https://ncbi.nlm.nih.gov/pubmed/24507611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.12.024
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