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Tethered Particle Motion Reveals that LacI·DNA Loops Coexist with a Competitor-Resistant but Apparently Unlooped Conformation

The lac repressor protein (LacI) efficiently represses transcription of the lac operon in Escherichia coli by binding to two distant operator sites on the bacterial DNA and causing the intervening DNA to form a loop. We employed single-molecule tethered particle motion to observe LacI-mediated loop...

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書誌詳細
主要な著者: Revalee, Joel D., Blab, Gerhard A., Wilson, Henry D., Kahn, Jason D., Meiners, Jens-Christian
フォーマット: Artigo
言語:Inglês
出版事項: The Biophysical Society 2014
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3944957/
https://ncbi.nlm.nih.gov/pubmed/24507611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.12.024
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