Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase

Human granulocyte colony-stimulating factor (hGCSF), a neutrophil-promoting cytokine, is an effective therapeutic agent for neutropenia patients who have undergone several cancer treatments. Efficient production of hGCSF using E. coli is challenging because the hormone tends to aggregate and forms i...

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Bibliografiske detaljer
Main Authors: Do, Bich Hang, Ryu, Han-Bong, Hoang, Phuong, Koo, Bon-Kyung, Choe, Han
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3940694/
https://ncbi.nlm.nih.gov/pubmed/24594699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0089906
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