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Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
Human fibroblast growth factor 21 (hFGF21) has been characterized as an important regulator of glucose and lipid metabolism homeostasis. Here, to produce hFGF21 efficiently in Escherichia coli, the expression and solubility of hFGF21 were tested and optimised by fusing the protein with one of eight...
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Publicado no: | Sci Rep |
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Main Authors: | , , , , , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Nature Publishing Group UK
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5700921/ https://ncbi.nlm.nih.gov/pubmed/29170489 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16167-x |
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