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Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein

Human fibroblast growth factor 21 (hFGF21) has been characterized as an important regulator of glucose and lipid metabolism homeostasis. Here, to produce hFGF21 efficiently in Escherichia coli, the expression and solubility of hFGF21 were tested and optimised by fusing the protein with one of eight...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Nguyen, Anh Ngoc, Song, Jung-A, Nguyen, Minh Tan, Do, Bich Hang, Kwon, Grace G., Park, Sang Su, Yoo, Jiwon, Jang, Jaepyeong, Jin, Jonghwa, Osborn, Mark J., Jang, Yeon Jin, Thi Vu, Thu Trang, Oh, Heung-Bum, Choe, Han
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5700921/
https://ncbi.nlm.nih.gov/pubmed/29170489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16167-x
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