Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase

Human granulocyte colony-stimulating factor (hGCSF), a neutrophil-promoting cytokine, is an effective therapeutic agent for neutropenia patients who have undergone several cancer treatments. Efficient production of hGCSF using E. coli is challenging because the hormone tends to aggregate and forms i...

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Detaylı Bibliyografya
Asıl Yazarlar: Do, Bich Hang, Ryu, Han-Bong, Hoang, Phuong, Koo, Bon-Kyung, Choe, Han
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2014
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3940694/
https://ncbi.nlm.nih.gov/pubmed/24594699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0089906
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