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Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.

Two 19-base-long oligonucleotides were synthesized, one complementary to the normal human beta-globin gene (beta A) and one complementary to the sickle cell beta-globin gene (beta S). The nonadecanucleotides were radioactively labeled and used as probes in DNA hybridization. Under appropriate hybrid...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Conner, B J, Reyes, A A, Morin, C, Itakura, K, Teplitz, R L, Wallace, R B
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393356/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6572002/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.1.278
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