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Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli.
The lacY gene of Escherichia coli, which encodes the lac permease, has been modified by oligonucleotide-directed, site-specific mutagenesis such that each of the four histidine residues in the molecule is replaced with an arginine residue. Replacement of histidine-35 and histidine-39 with arginine h...
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| Udgivet i: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390767/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3901007/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.20.6765 |
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