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Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli.

The lacY gene of Escherichia coli, which encodes the lac permease, has been modified by oligonucleotide-directed, site-specific mutagenesis such that each of the four histidine residues in the molecule is replaced with an arginine residue. Replacement of histidine-35 and histidine-39 with arginine h...

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Dettagli Bibliografici
Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Padan, E, Sarkar, H K, Viitanen, P V, Poonian, M S, Kaback, H R
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1985
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390767/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3901007/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.20.6765
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