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Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli.

The lacY gene of Escherichia coli, which encodes the lac permease, has been modified by oligonucleotide-directed, site-specific mutagenesis such that each of the four histidine residues in the molecule is replaced with an arginine residue. Replacement of histidine-35 and histidine-39 with arginine h...

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Détails bibliographiques
Auteurs principaux: Padan, E, Sarkar, H K, Viitanen, P V, Poonian, M S, Kaback, H R
Format: Artigo
Langue:Inglês
Publié: 1985
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC390767/
https://ncbi.nlm.nih.gov/pubmed/3901007
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