Histidine-94 is the only important histidine residue in the melibiose permease of Escherichia coli.
Oligonucleotide-directed, site-specific mutagenesis has been utilized to modify the melB gene of Escherichia coli such that each of the seven His residues in the melibiose permease has been replaced with Arg. Replacement of His-213, His-442, or His-456 has no significant effect on permease activity,...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53285/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2404282/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.1.468 |
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