In vivo expression of the lacY gene in two segments leads to functional lac permease.
The lacY gene of Escherichia coli was cut into two approximately equal-size fragments with Afl II and subcloned individually or together under separate lac operator/promoters in plasmid pT7-5. Under these conditions, lac permease is expressed in two portions: (i) the N-terminal portion (the N termin...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54102/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2190220/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.11.4325 |
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