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In vivo expression of the lacY gene in two segments leads to functional lac permease.

The lacY gene of Escherichia coli was cut into two approximately equal-size fragments with Afl II and subcloned individually or together under separate lac operator/promoters in plasmid pT7-5. Under these conditions, lac permease is expressed in two portions: (i) the N-terminal portion (the N termin...

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Bibliographic Details
Main Authors: Bibi, E, Kaback, H R
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC54102/
https://ncbi.nlm.nih.gov/pubmed/2190220
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