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Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System

Nonviral gene delivery systems are rapidly becoming a desirable and applicable method to overexpress genes in various types of cells. We have recently developed a piggyBac transposase-based, helper-independent and self-inactivating delivery system (pmGENIE-3) capable of high-efficiency transfection...

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Bibliografiske detaljer
Main Authors: Owens, Jesse B, Mathews, Juanita, Davy, Philip, Stoytchev, Ilko, Moisyadi, Stefan, Allsopp, Rich
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3894583/
https://ncbi.nlm.nih.gov/pubmed/24326734
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtna.2013.61
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