Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis

We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by five...

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Bibliografiske detaljer
Main Authors: Marh, Joel, Stoytcheva, Zoia, Urschitz, Johann, Sugawara, Atsushi, Yamashiro, Hideaki, Owens, Jesse B., Stoytchev, Ilko, Pelczar, Pawel, Yanagimachi, Ryuzo, Moisyadi, Stefan
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2012
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3511126/
https://ncbi.nlm.nih.gov/pubmed/23093669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1216473109
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