Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis

We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by five...

Полное описание

Сохранить в:
Библиографические подробности
Главные авторы: Marh, Joel, Stoytcheva, Zoia, Urschitz, Johann, Sugawara, Atsushi, Yamashiro, Hideaki, Owens, Jesse B., Stoytchev, Ilko, Pelczar, Pawel, Yanagimachi, Ryuzo, Moisyadi, Stefan
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 2012
Предметы:
Biological Sciences
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3511126/
https://ncbi.nlm.nih.gov/pubmed/23093669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1216473109
Метки: Добавить метку
Нет меток, Требуется 1-ая метка записи!

Схожие документы