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High-resolution melt analysis to detect sequence variations in highly homologous gene regions: application to CYP2B6

High-resolution melt (HRM) analysis using ‘release-on-demand’ dyes, such as EvaGreen(®) has the potential to resolve complex genotypes in situations where genotype interpretation is complicated by the presence of pseudogenes or allelic variants in close proximity to the locus of interest. We explore...

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Detalhes bibliográficos
Main Authors: Twist, Greyson P, Gaedigk, Roger, Leeder, J Steven, Gaedigk, Andrea
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3866959/
https://ncbi.nlm.nih.gov/pubmed/23746185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2217/pgs.13.66
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