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A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli
To site-specifically incorporate an unnatural amino acid (UAA) into target proteins in Escherichia coli, we use a suppressor plasmid that provides an engineered suppressor tRNA and an aminoacyl-tRNA synthetase (aaRS) specific for the UAA of interest. The continuous drive to further improve UAA incor...
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| Hlavní autoři: | , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2013
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3855549/ https://ncbi.nlm.nih.gov/pubmed/23379331 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi4000244 |
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