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Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

Here we report the development of a baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells. We have exploited the large cargo-capacity (>30 kb) and stability of the double-stranded DNA genome of baculovirus to deliver t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chatterjee, Abhishek, Xiao, Han, Bollong, Michael, Ai, Hui-Wang, Schultz, Peter G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3718144/
https://ncbi.nlm.nih.gov/pubmed/23818609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1309584110
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