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An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli

The addition of novel amino acids to the genetic code of Escherichia coli involves the generation of an aminoacyl-tRNA synthetase and tRNA pair that is ‘orthogonal’, meaning that it functions independently of the synthetases and tRNAs endogenous to E.coli. The amino acid specificity of the orthogona...

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Detalhes bibliográficos
Main Authors: Santoro, Stephen W., Anderson, J. Christopher, Lakshman, Vishva, Schultz, Peter G.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC290271/
https://ncbi.nlm.nih.gov/pubmed/14627803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkg903
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