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Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

[Image: see text] We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNA(CUA)(Pyl) pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae. In the process we have solved the key challenges of producing a functiona...

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Detalhes bibliográficos
Main Authors: Hancock, Susan M., Uprety, Rajendra, Deiters, Alexander, Chin, Jason W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2010
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2956376/
https://ncbi.nlm.nih.gov/pubmed/20925334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja104609m
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