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Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair
[Image: see text] We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNA(CUA)(Pyl) pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae. In the process we have solved the key challenges of producing a functiona...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Chemical Society
2010
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2956376/ https://ncbi.nlm.nih.gov/pubmed/20925334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja104609m |
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