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Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo
In an effort to expand the scope of protein mutagenesis, we have completed the first steps toward a general method to allow the site-specific incorporation of unnatural amino acids into proteins in vivo. Our approach involves the generation of an “orthogonal” suppressor tRNA that is uniquely acylate...
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| Main Authors: | , , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
The National Academy of Sciences of the USA
1997
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC23315/ https://ncbi.nlm.nih.gov/pubmed/9294168 |
| Clibeanna: |
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