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Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio
The ATP:ADP ratio is a critical parameter of cellular energy status that regulates many metabolic activities. Here we report an optimized genetically-encoded fluorescent biosensor, PercevalHR, that senses the ATP:ADP ratio. PercevalHR is tuned to the range of intracellular ATP:ADP expected in mammal...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3852917/ https://ncbi.nlm.nih.gov/pubmed/24096541 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms3550 |
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