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Quantitative two-photon imaging of fluorescent biosensors

Fluorescent biosensors are now routinely imaged using two-photon microscopy in intact tissue, for instance, in brain slices and brains in living animals. But most studies measure temporal variation – e.g., calcium transients in response to neuronal activity – rather than calibrated levels of biosens...

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Detalhes bibliográficos
Publicado no:Curr Opin Chem Biol
Main Authors: Yellen, Gary, Mongeon, Rebecca
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4553104/
https://ncbi.nlm.nih.gov/pubmed/26079046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cbpa.2015.05.024
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