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Kinetic analysis of cytokine-mediated receptor assembly using engineered FC heterodimers
A method for analyzing ligand–receptor binding kinetics is described, which is based on an engineered FC domain (FChk) that forms a covalent heterodimer. To validate the system, the type I IFN receptors (IFNAR1 and IFNAR2) were expressed as IFNAR1-FChk, IFNAR2-FCkh, and IFNAR1/IFNAR2-FChk fusion pro...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Blackwell Publishing Ltd
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3832046/ https://ncbi.nlm.nih.gov/pubmed/23703950 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2285 |
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