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Role of Lysine 411 in Substrate Carboxyl Group Binding to the Human Reduced Folate Carrier, as Determined by Site-directed Mutagenesis and Affinity Inhibition
Reduced folate carrier (RFC) is the major membrane transporter for folates and antifolates in mammalian tissues. Recent studies used radioaffinity labeling with N-hydroxysuccinimide (NHS)-(3)H-methotrexate (MTX) to localize substrate binding to residues in transmembrane domain (TMD) 11 of human RFC....
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3806200/ https://ncbi.nlm.nih.gov/pubmed/18182479 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/mol.107.043190 |
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