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Role of Lysine 411 in Substrate Carboxyl Group Binding to the Human Reduced Folate Carrier, as Determined by Site-directed Mutagenesis and Affinity Inhibition

Reduced folate carrier (RFC) is the major membrane transporter for folates and antifolates in mammalian tissues. Recent studies used radioaffinity labeling with N-hydroxysuccinimide (NHS)-(3)H-methotrexate (MTX) to localize substrate binding to residues in transmembrane domain (TMD) 11 of human RFC....

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Autors principals: Deng, Yijun, Hou, Zhanjun, Wang, Lei, Cherian, Christina, Wu, Jianmei, Gangjee, Aleem, Matherly, Larry H.
Format: Artigo
Idioma:Inglês
Publicat: 2008
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3806200/
https://ncbi.nlm.nih.gov/pubmed/18182479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/mol.107.043190
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