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The Effect of Ritonavir on Human CYP2B6 Catalytic Activity: Heme Modification Contributes to the Mechanism-Based Inactivation of CYP2B6 and CYP3A4 by Ritonavir

The mechanism-based inactivation of human CYP2B6 by ritonavir (RTV) in a reconstituted system was investigated. The inactivation is time, concentration, and NADPH dependent and exhibits a K(I) of 0.9 μM, a k(inact) of 0.05 min(−1), and a partition ratio of approximately 3. Liquid chromatography–tand...

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Dettagli Bibliografici
Autori principali: Lin, Hsia-lien, D’Agostino, Jaime, Kenaan, Cesar, Calinski, Diane, Hollenberg, Paul F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Pharmacology and Experimental Therapeutics 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3781371/
https://ncbi.nlm.nih.gov/pubmed/23886699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/dmd.113.053108
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