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Reversible protein affinity-labelling using bromomaleimide-based reagents
Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild c...
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| Hauptverfasser: | , , , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Royal Society of Chemistry
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3763775/ https://ncbi.nlm.nih.gov/pubmed/23462873 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3ob40239h |
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