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Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
A simple and robust method for targeted mutagenesis in zebrafish has long been sought. Previous methods generate monoallelic mutations in the germ line of F0 animals, usually delaying homozygosity for the mutation to the F2 generation. Generation of robust biallelic mutations in the F0 would allow f...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2013
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3752207/ https://ncbi.nlm.nih.gov/pubmed/23918387 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1308335110 |
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