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Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

A simple and robust method for targeted mutagenesis in zebrafish has long been sought. Previous methods generate monoallelic mutations in the germ line of F0 animals, usually delaying homozygosity for the mutation to the F2 generation. Generation of robust biallelic mutations in the F0 would allow f...

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Detalhes bibliográficos
Main Authors: Jao, Li-En, Wente, Susan R., Chen, Wenbiao
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3752207/
https://ncbi.nlm.nih.gov/pubmed/23918387
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1308335110
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