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High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

[Image: see text] Actinobacteria, particularly those of genus Streptomyces, remain invaluable hosts for the discovery and engineering of natural products and their cognate biosynthetic pathways. However, genetic manipulation of these bacteria is often labor and time intensive. Here, we present an en...

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Vydáno v:ACS Synth Biol
Hlavní autoři: Cobb, Ryan E., Wang, Yajie, Zhao, Huimin
Médium: Artigo
Jazyk:Inglês
Vydáno: American Chemical Society 2014
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4459934/
https://ncbi.nlm.nih.gov/pubmed/25458909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb500351f
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