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High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

[Image: see text] Actinobacteria, particularly those of genus Streptomyces, remain invaluable hosts for the discovery and engineering of natural products and their cognate biosynthetic pathways. However, genetic manipulation of these bacteria is often labor and time intensive. Here, we present an en...

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Publicat a:ACS Synth Biol
Autors principals: Cobb, Ryan E., Wang, Yajie, Zhao, Huimin
Format: Artigo
Idioma:Inglês
Publicat: American Chemical Society 2014
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4459934/
https://ncbi.nlm.nih.gov/pubmed/25458909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb500351f
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