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High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

[Image: see text] Actinobacteria, particularly those of genus Streptomyces, remain invaluable hosts for the discovery and engineering of natural products and their cognate biosynthetic pathways. However, genetic manipulation of these bacteria is often labor and time intensive. Here, we present an en...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:ACS Synth Biol
Main Authors: Cobb, Ryan E., Wang, Yajie, Zhao, Huimin
Format: Artigo
Jezik:Inglês
Izdano: American Chemical Society 2014
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4459934/
https://ncbi.nlm.nih.gov/pubmed/25458909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb500351f
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