High-throughput analysis of type I-E CRISPR/Cas spacer acquisition in E. coli

In Escherichia coli, the acquisition of new CRISPR spacers is strongly stimulated by a priming interaction between a spacer in CRISPR RNA and a protospacer in foreign DNA. Priming also leads to a pronounced bias in DNA strand from which new spacers are selected. Here, ca. 200,000 spacers acquired du...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Savitskaya, Ekaterina, Semenova, Ekaterina, Dedkov, Vladimir, Metlitskaya, Anastasia, Severinov, Konstantin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Landes Bioscience 2013
Schlagworte:
Research Paper
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3737330/
https://ncbi.nlm.nih.gov/pubmed/23619643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.24325
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge