High-throughput analysis of type I-E CRISPR/Cas spacer acquisition in E. coli

In Escherichia coli, the acquisition of new CRISPR spacers is strongly stimulated by a priming interaction between a spacer in CRISPR RNA and a protospacer in foreign DNA. Priming also leads to a pronounced bias in DNA strand from which new spacers are selected. Here, ca. 200,000 spacers acquired du...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Savitskaya, Ekaterina, Semenova, Ekaterina, Dedkov, Vladimir, Metlitskaya, Anastasia, Severinov, Konstantin
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Landes Bioscience 2013
Pynciau:
Research Paper
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3737330/
https://ncbi.nlm.nih.gov/pubmed/23619643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.24325
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