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High-throughput analysis of type I-E CRISPR/Cas spacer acquisition in E. coli
In Escherichia coli, the acquisition of new CRISPR spacers is strongly stimulated by a priming interaction between a spacer in CRISPR RNA and a protospacer in foreign DNA. Priming also leads to a pronounced bias in DNA strand from which new spacers are selected. Here, ca. 200,000 spacers acquired du...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3737330/ https://ncbi.nlm.nih.gov/pubmed/23619643 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.24325 |
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