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Determination of detection and quantification limits for SNP allele frequency estimation in DNA pools using real time PCR

The quantification of single nucleotide polymorphism (SNP) allele frequencies in pooled DNA samples using real time PCR is a promising approach for large-scale diagnostics and genotyping. The limits of detection (LOD) and limits of quantification (LOQ) for mutant SNP alleles are of particular import...

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Detalhes bibliográficos
Main Authors: Schwarz, Gerhard, Bäumler, Stefan, Block, Annette, Felsenstein, Friedrich G., Wenzel, Gerhard
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC373417/
https://ncbi.nlm.nih.gov/pubmed/14960708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh020
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