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Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry

We report here the design, synthesis and evaluation of a novel photocleavable (PC) biotinylated nucleotide analog, dUTP-PC-Biotin, for DNA polymerase extension reaction to isolate DNA products for mass spectrometry (MS) analysis. This nucleotide analog has a biotin moiety attached to the 5-position...

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Main Authors: Bai, Xiaopeng, Kim, Sobin, Li, Zengmin, Turro, Nicholas J., Ju, Jingyue
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC373325/
https://ncbi.nlm.nih.gov/pubmed/14744978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh198
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