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Guidelines for incorporating non-perfectly matched oligonucleotides into target-specific hybridization probes for a DNA microarray
Sequence-specific oligonucleotide probes play a crucial role in hybridization techniques including PCR, DNA microarray and RNA interference. Once the entire genome becomes the search space for target genes/genomic sequences, however, cross-hybridization to non-target sequences becomes a problem. Lar...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373323/ https://ncbi.nlm.nih.gov/pubmed/14757833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh196 |
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