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Guidelines for incorporating non-perfectly matched oligonucleotides into target-specific hybridization probes for a DNA microarray

Sequence-specific oligonucleotide probes play a crucial role in hybridization techniques including PCR, DNA microarray and RNA interference. Once the entire genome becomes the search space for target genes/genomic sequences, however, cross-hybridization to non-target sequences becomes a problem. Lar...

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Hlavní autoři: Lee, Inhan, Dombkowski, Alan A., Athey, Brian D.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC373323/
https://ncbi.nlm.nih.gov/pubmed/14757833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh196
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